biomaterial

Allevi Author: Brigham & Women's Hospital Proves Porous is Preferred

2018-aqueous-two-phase-emulsion-bioink-enabled-3d-bioprinting-of-porous-hydrogels-cover allevi in 3d bioprinter

We’re so excited to welcome the Yu Shrike Zhang lab from Brigham & Women’s Hospital to the Allevi Author Club!

3D bioprinting is an amazing technology which allows researchers to create custom cell-laden constructs that mimic the human body better than their 2D counterparts. Here at Allevi, our mission is to make it easy for scientists to replicate the body outside the body. Our community of users is composed of the leadings minds in tissue engineering and they are working on every type of tissue from brain to bone.

Agnostic of tissue type, one of most important aspects of 3d bioprinting is ensuring that your cells organize and proliferate as they would in your body. Bioinks provide cells with a much needed support that allows them to more easily organize into the geometries that they would in native tissue. However, if a bioink is too dense or too rigid, it can actually hinder the proliferation of cells and prevent them from performing their needed function.

Our new #AlleviAuthors tackled this problem in their new paper titled “Aqueous Two‐Phase Emulsion Bioink‐Enabled 3D Bioprinting of Porous Hydrogels” and published in Advanced Materials.

By creating an aqueous bioink emulsion, the researchers were able to create a construct that is porous in composition while at the same time providing the rigidity needed in order to create 3D constructs. Their bioink is composed of cells mixed with GelMA and PEO which are immiscible materials - meaning that they do not mix in a homogenous manner. A classic example of immiscible liquids is oil and water. The fact that GelMA and PEO naturally repel each other means that small droplets of each material exist side by side within the bioink.

Using the Allevi 2 bioprinters, this bioink was bioprinted and crosslinked to form the desired geometry and rigidity of the tissue type that you are recreating. After the desired geometry has been achieved, you are then able to remove the PEO from the construct leaving small holes in the structure that allow cells to proliferate with greater ease.

The researchers tested their new method across 3 different cell lines and found that the porous 3D-bioprinted hydrogels showed enhanced cell viability and proliferation vs nonporous hydrogels. This new method means that researchers across any tissue type are now able to create porous-structures with higher cell viability. We’re excited to see the FAR reaching effects of this method for our entire community of Allevi researchers!

Read on to learn more about their novel bioink and how to incorporate it into your research: https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201805460

Allevi Author: NJIT Bioprints Vascularized Tissue

NJIT allevi guvendiren vascular vasculature vein 3d bioprint bioprinted bioprinter

We are VERY excited to announce the latest addition the Allevi Author Club; the Guvendiren Lab from the New Jersey Institute of Technology.

Dr. Guvendiren’s lab focuses on creating novel bioinks for tissue engineering and regenerative medicine applications with a focus on 3D bioprinting. Their most recent paper, published in Acta Biomaterialia and titled “3D bioprinting of complex channels within cell-laden hydrogels”, explores their new approach to 3D bioprinting vasculature into 3D tissue.

There are many different methods for creating microchannels within constructs, including electrospinning, fiber bonding, and casting solvents into molds. However these techniques don’t allow for precise control of channel size, shape or location. They can also be time-consuming and restrictive in the number of cell lines that you are able to work with simultaneously.

The Guvendiren lab is exploring a new approach to creating these channel-laden tissues using their Allevi 2 bioprinter. In their paper, they explore a method of 3D bioprinting sacrificial bioinks into cell-laden hydrogels (pluronic into methacrylated alginate/methacrylated hyaluronic acid to be specific). This technique allows them to create custom channel geometries, control channel thickness and tune the hydrogel rigidity. They also explored a super cool technique wherein they alter the printhead speed in order to create channels of differing diameters.

Their images from confocal scanning show strong endothelial cell (HUVEC) attachment to the channel walls and depict the final 3D bioprinted vein construct.

HUVEC vascular channel vein 3d bioprinted bioprint allevi NJIT guvendiren

This research explores important techniques for creating tunable microchannels within 3D tissues. We can imagine a future wherein these methods are used to create 3D bioprinted organs with custom and complex vascular networks. It could also be used to create custom 3D models to study disease progression and test drug efficacy and toxicity. Amazing work, Guvendiren Lab!!

Click through to read their material characterization and learn more about their bioprinting approach: https://www.sciencedirect.com/science/article/pii/S1742706119301515.

The Allevi Coaxial Kit

We’re happy to announce the newest addition to our growing library of bioink kits - the Allevi Coxial Kit.

This new bioink kit allows users with an Allevi 2, Allevi 3 or Allevi 6 to mix materials from two syringes during the printing process. This is especially useful when working with materials that require curing catalysts or liquid crosslinking agents (i.e. sodium alginate, calcium chloride, certain silicones, etc).

The ability to mix materials at the nozzle opens up a whole new frontier of materials that you are able to extrude from your Allevi bioprinter. The Coaxial Kit is prepackaged with everything you need to get started out of the box including coaxial tip, tubing, luer lock tip connectors, and custom coaxial gcode.

Our mission here at Allevi is to supply you with best possible bioprinting tools that make it easy to bring your work to life. We are constantly testing new methods, bioinks, and tools in our lab to ensure that we are delivering cutting edge techniques to your bench. Together we are making giant strides in the field of tissue engineering and uncovering new methods that will forever change the way we #buildwithlife. We can’t wait to see what you will build with this one.