bioprint

Allevi Author: NJIT Bioprints Vascularized Tissue

NJIT allevi guvendiren vascular vasculature vein 3d bioprint bioprinted bioprinter

We are VERY excited to announce the latest addition the Allevi Author Club; the Guvendiren Lab from the New Jersey Institute of Technology.

Dr. Guvendiren’s lab focuses on creating novel bioinks for tissue engineering and regenerative medicine applications with a focus on 3D bioprinting. Their most recent paper, published in Acta Biomaterialia and titled “3D bioprinting of complex channels within cell-laden hydrogels”, explores their new approach to 3D bioprinting vasculature into 3D tissue.

There are many different methods for creating microchannels within constructs, including electrospinning, fiber bonding, and casting solvents into molds. However these techniques don’t allow for precise control of channel size, shape or location. They can also be time-consuming and restrictive in the number of cell lines that you are able to work with simultaneously.

The Guvendiren lab is exploring a new approach to creating these channel-laden tissues using their Allevi 2 bioprinter. In their paper, they explore a method of 3D bioprinting sacrificial bioinks into cell-laden hydrogels (pluronic into methacrylated alginate/methacrylated hyaluronic acid to be specific). This technique allows them to create custom channel geometries, control channel thickness and tune the hydrogel rigidity. They also explored a super cool technique wherein they alter the printhead speed in order to create channels of differing diameters.

Their images from confocal scanning show strong endothelial cell (HUVEC) attachment to the channel walls and depict the final 3D bioprinted vein construct.

HUVEC vascular channel vein 3d bioprinted bioprint allevi NJIT guvendiren

This research explores important techniques for creating tunable microchannels within 3D tissues. We can imagine a future wherein these methods are used to create 3D bioprinted organs with custom and complex vascular networks. It could also be used to create custom 3D models to study disease progression and test drug efficacy and toxicity. Amazing work, Guvendiren Lab!!

Click through to read their material characterization and learn more about their bioprinting approach: https://www.sciencedirect.com/science/article/pii/S1742706119301515.

The Allevi Coaxial Kit

We’re happy to announce the newest addition to our growing library of bioink kits - the Allevi Coxial Kit.

This new bioink kit allows users with an Allevi 2, Allevi 3 or Allevi 6 to mix materials from two syringes during the printing process. This is especially useful when working with materials that require curing catalysts or liquid crosslinking agents (i.e. sodium alginate, calcium chloride, certain silicones, etc).

The ability to mix materials at the nozzle opens up a whole new frontier of materials that you are able to extrude from your Allevi bioprinter. The Coaxial Kit is prepackaged with everything you need to get started out of the box including coaxial tip, tubing, luer lock tip connectors, and custom coaxial gcode.

Our mission here at Allevi is to supply you with best possible bioprinting tools that make it easy to bring your work to life. We are constantly testing new methods, bioinks, and tools in our lab to ensure that we are delivering cutting edge techniques to your bench. Together we are making giant strides in the field of tissue engineering and uncovering new methods that will forever change the way we #buildwithlife. We can’t wait to see what you will build with this one.

Bioprinting in the News: 'Bioprinters Are Churning Out Living Fixes to Broken Spines' By WIRED

Image courtesy of www.wired.com

Image courtesy of www.wired.com

Bioprinters are an essential piece of lab equipment for any scientist, researcher, or doctor that wants to study cells in a relevant way. This is because cells in 3D behave differently than their counterparts studied in a 2D environment; they express more accurate biomarkers and perform more physiologically relevant actions. Bioprinters accelerate the pace of research and allow scientists to find innovative solutions to real world problems.

This awesome article by WIRED profiles a team at UC San Diego that has bioprinted a section of spinal cord that can be custom-fit into a patient’s injury.

It’s awesome to see how bioprinting allows researchers to reliably study the body outside the body. Together, we can change the way we study and treat illness!

Read the full article here.

The Allevi Academy

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By Lauren McLeod, Bioengineer: 

Here at Allevi, we’re always looking to the future - how to prepare for future challenges, how to revolutionize and improve on current research and methodologies...but sometimes it’s necessary to reflect on the past.  We took some time to think about the education experiences that got us to where we are today. Most of us conjured up memories of an impressionable teacher, exciting project, or even an awesome field trip that sparked an excitement for learning and science.  We thought to ourselves, “Why not have bioprinting be the seed of students’ excitement and learning for the field of bioengineering?”

We’re excited to announce the launch of The Allevi Academy- the first step in preparing today’s students for the regenerative medicine challenges of the future!  We partnered with high school teachers, university professors, and educators across the world to produce the best, most streamlined and accessible curriculum possible to arm teachers with the materials and resources needed to introduce their students to bioprinting.  

Through our curriculum, students gain experience with cutting edge biotechnology, putting them light years ahead of their peers as they enter college and the workforce.  According to the US Bureau of Labor Statistics, bioengineers hold the third fastest growing job in the United States, with a projected ten year growth of 61.7% by 2020. Our curriculum gives students a competitive advantage in this burgeoning field.

The curriculum enables students to develop valuable skills across multiple engineering disciplines. Included activities incorporate coding, computer aided design, engineering drawings and 3D fabrication to produce innovative solutions for situations modeled after real life tissue engineering challenges. From designing and prototyping hydrogel wound coverings, to vascularization channels for organ on a chip applications, students learn to problem solve and think critically- skills that span way beyond the field of bioengineering.

       -    All inclusive
       -    Easy to use
       -    Satisfies Next Generation Science 
       -    Satisfies National Science Education Standards
       -    Hands on
       -    Real world applications
       -    Adaptable

Check out The Allevi Academy and learn how you can prepare students for the future of STEM and provide them with the tools they need to tackle the challenges of the future!

Print Alive, Print Allevi

Allevi Joins Johnson&Johnson's JPOD Philadelphia Community

As new applications continue to be developed within the Allevi community, we feel the responsibility to join larger ecosystems that connect us with more engineers, scientists, and physicians that can benefit from the power of Allevi’s solutions. Our first successful relationship with a broader ecosystem has been with VWR. They continue to help us introduce our platform to accelerate science around the world. Adding geometry to biology is becoming a standard to be able to generate accurate representations of the body outside the body. 

At Allevi, we are now ready to begin to understand how to translate our communities’ scientific discoveries into the breakthroughs of tomorrow. To do this we realize that we need to co-create solutions with those at the front lines who are bringing new drugs to market, fighting diseases at the bedside, and producing novel solutions for the healthcare system.

Therefore, we are excited to join the Johnson & Johnson Innovation, JLABS JPOD @ Philadelphia community. We believe access to this ecosystem helps us connect with those who understand how Allevi’s applications can serve the healthcare industry. These interactions will guide our community today and in the future. Together we can target healthcare’s biggest needs by creating more accurate, predictable 3D biology models.

As we continue to collaborate with the Johnson & Johnson Innovation community, we will look forward to receiving mentorship, brainstorming with great minds in the industry, and continuing to grow Allevi. It takes a village to raise a company and we are happy to have the JLABS village help us reach new heights.