veins

Allevi Author: NJIT Bioprints Vascularized Tissue

NJIT allevi guvendiren vascular vasculature vein 3d bioprint bioprinted bioprinter

We are VERY excited to announce the latest addition the Allevi Author Club; the Guvendiren Lab from the New Jersey Institute of Technology.

Dr. Guvendiren’s lab focuses on creating novel bioinks for tissue engineering and regenerative medicine applications with a focus on 3D bioprinting. Their most recent paper, published in Acta Biomaterialia and titled “3D bioprinting of complex channels within cell-laden hydrogels”, explores their new approach to 3D bioprinting vasculature into 3D tissue.

There are many different methods for creating microchannels within constructs, including electrospinning, fiber bonding, and casting solvents into molds. However these techniques don’t allow for precise control of channel size, shape or location. They can also be time-consuming and restrictive in the number of cell lines that you are able to work with simultaneously.

The Guvendiren lab is exploring a new approach to creating these channel-laden tissues using their Allevi 2 bioprinter. In their paper, they explore a method of 3D bioprinting sacrificial bioinks into cell-laden hydrogels (pluronic into methacrylated alginate/methacrylated hyaluronic acid to be specific). This technique allows them to create custom channel geometries, control channel thickness and tune the hydrogel rigidity. They also explored a super cool technique wherein they alter the printhead speed in order to create channels of differing diameters.

Their images from confocal scanning show strong endothelial cell (HUVEC) attachment to the channel walls and depict the final 3D bioprinted vein construct.

HUVEC vascular channel vein 3d bioprinted bioprint allevi NJIT guvendiren

This research explores important techniques for creating tunable microchannels within 3D tissues. We can imagine a future wherein these methods are used to create 3D bioprinted organs with custom and complex vascular networks. It could also be used to create custom 3D models to study disease progression and test drug efficacy and toxicity. Amazing work, Guvendiren Lab!!

Click through to read their material characterization and learn more about their bioprinting approach: https://www.sciencedirect.com/science/article/pii/S1742706119301515.

Allevi Author: 3D‐Printed Sugar Stents to Aid in Surgery

Microvascular anastomosis (or the method of surgically connecting blood vessels) is a common part of many reconstructive and transplant surgical procedures.

There are multiple methods for connecting two veins together including coupling devices, surgical glue, and surgical suturing but each method has it’s downsides; coupling devices can face rejection from the body, glue can introduce contamination or clotting to the vein, and suturing (the most commonly accepted practice) is a delicate and time consuming procedure.

 
suturing blood vessels
 

During the suturing procedure, surgeons are in a race against the clock to quickly connect the veins together to ensure that organs continue to receive proper blood flow. However, blood vessels of differing shapes and sizes can sometimes make this procedure difficult to maneuver in a timely fashion.

 
allevi author 3d bioprinted sugar stents to aid in surgical suturing.jpg
 

In their recent paper titled, “3D‐Printed Sugar‐Based Stents Facilitating Vascular Anastomosis”, researchers at Brigham and Women’s Hospital & The University of Nebraska Lincoln collaborated using an Allevi 2 bioprinter to find a solution to aid in the intricacies surrounding this procedure.

Here, dissolvable sugar‐based stents are 3D printed as an assistive tool for facilitating surgical anastomosis. The non-brittle sugar‐based stent holds the vessels together during the procedure and are dissolved upon the restoration of the blood flow. The incorporation of sodium citrate minimizes the chance of thrombosis, and the dissolution rate of the sugar‐based stent can be tailored between 4 and 8 min.

 
allevi 2 3d bioprinter fabricates sugar stents to aid in surgical procedure
 

3D printing is an ideal method for constructing these stents because you are able to quickly design and create custom geometries to fit the patient’s vessels.

The effectiveness of the printed sugar‐based stent was assessed ex vivo and found to be a fast and reliable fabrication method that can be performed in the operating room.

This new method of aiding surgeons is a game-changer as it is dissolvable, tunable, and completely customizable. In the future, your doctor could quickly print out stents to match your exact vein geometry which would reduce the time spent on the operating table and under anesthesia.

Interested in learning more about this novel technique? You can read the full paper here: https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.201800702?af=R&

10 Cools Things You Could Print with a 3D Bioprinter in the Near Future

3D bioprinting is an intuitive way to approach biology. But not many people realize its versatility. To give an idea of what is possible through 3D bioprinting, we’re starting a little series called “Allevi Applications.” Hopefully, this will make the idea of bioprinting a little more accessible! So without further ado, let’s get started.

1. Joint replacements, think knee, ankle and elbow.

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2. Microfluidic chips

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3. Cell scaffolds for replacement organs, eventually making fulling functioning organs

3. cell scaffolds for replacement parts.jpg

4. Cartilage

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5. Accurate surgical models for physicians to practice difficult procedures

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6. Drugs with custom release rates, compositions and geometries

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7. Teeth and dental implants

8. Skin grafts for burn victims

9. Casts and bioactive clothing

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10.  Blood vessels, arteries and heart valves

And our users are just getting started. Check back as we cover new publications from #Allevi Authors and see what amazing applications they come up with next.