Allevi Applications

The Allevi Academy

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By Lauren McLeod, Bioengineer: 

Here at Allevi, we’re always looking to the future - how to prepare for future challenges, how to revolutionize and improve on current research and methodologies...but sometimes it’s necessary to reflect on the past.  We took some time to think about the education experiences that got us to where we are today. Most of us conjured up memories of an impressionable teacher, exciting project, or even an awesome field trip that sparked an excitement for learning and science.  We thought to ourselves, “Why not have bioprinting be the seed of students’ excitement and learning for the field of bioengineering?”

We’re excited to announce the launch of The Allevi Academy- the first step in preparing today’s students for the regenerative medicine challenges of the future!  We partnered with high school teachers, university professors, and educators across the world to produce the best, most streamlined and accessible curriculum possible to arm teachers with the materials and resources needed to introduce their students to bioprinting.  

Through our curriculum, students gain experience with cutting edge biotechnology, putting them light years ahead of their peers as they enter college and the workforce.  According to the US Bureau of Labor Statistics, bioengineers hold the third fastest growing job in the United States, with a projected ten year growth of 61.7% by 2020. Our curriculum gives students a competitive advantage in this burgeoning field.

The curriculum enables students to develop valuable skills across multiple engineering disciplines. Included activities incorporate coding, computer aided design, engineering drawings and 3D fabrication to produce innovative solutions for situations modeled after real life tissue engineering challenges. From designing and prototyping hydrogel wound coverings, to vascularization channels for organ on a chip applications, students learn to problem solve and think critically- skills that span way beyond the field of bioengineering.

       -    All inclusive
       -    Easy to use
       -    Satisfies Next Generation Science 
       -    Satisfies National Science Education Standards
       -    Hands on
       -    Real world applications
       -    Adaptable

Check out The Allevi Academy and learn how you can prepare students for the future of STEM and provide them with the tools they need to tackle the challenges of the future!

Print Alive, Print Allevi

Cosmetic Animal Testing is on its Way Out

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The fight to ban animal testing recently scored a major victory when California became the first US state to pass a bill that would ban testing cosmetics on animals.

Companies conduct animal tests to determine the safety of new formulas used in cosmetic products. These tests include skin and eye irritation tests to study allergic reactions and “lethal-dose” testing to determine ingredient toxicity.

But these tests have scientific limitations because humans and animals react differently to certain chemicals. Therefore these tests, that are meant to study the safety of cosmetics before they touch human skin, aren’t even relevant.

Here at Allevi, we believe in the future of personalized cosmetic testing. Our 3D bioprinters allow researchers to customize every aspect of their study - including tissue shape, size, geometry and cell line. Imagine a world where drugs and cosmetics are no longer cruelly tested on animals, but tested on patches of human tissue. But not just any human’s tissue but YOURS because you are different from a rabbit and different from a monkey and even different from your fellow human.

We applaud California for following in the EU, Norway, Israel and India’s footsteps. It’s time we replace these outdated methods; not only because they are cruel to animals but because they are ineffective. Allevi is here to usher in a new era for a cruelty free and scientifically relevant future.

Allevi Author: Plant Based Hydrogels for Cell Laden Bioprinting

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Time for another inductee to the #AlleviAuthor club. Researchers from University of California, Berkeley and IBM used their Allevi 2 bioprinter to study the printability and viability of plant based bioinks.

In their paper titled, “Agarose-Based Hydrogels as Suitable Bioprinting Materials for Tissue Engineering” and published in ACS Biomaterials Science & Engineering, they compared agarose-based hydrogels commonly used for cartilage tissue engineering to Pluronic. The goal is to find a bioink that has great printability without sacrificing cell viability.

The team compared mechanical and rheological properties, including yield stress, storage modulus, and shear thinning, as well as construct shape fidelity to assess their potential as a bioink for cell-based tissue engineering. Read on to find out which ratios of alginate and agarose demonstrated the best cell viability as well as print structure for their cartilage tissue engineering needs: https://cdn-pubs.acs.org/doi/10.1021/acsbiomaterials.8b00903.

Bioprinting offers hope of new treatment paths for cancer patients

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Our amazing users at The University of Waikato will use their Allevi 2 to research new treatment paths for cancer patients that could eventually lead to cancer tumors being treated outside patients' bodies.

We are constantly inspired by this amazing community of scientists who are changing the way we design, heal and build with life. And we're here to support them along the way! Read on to learn more about this incredible research.

The Challenges and Advances of Imitating Nature

Allevi Advanced Biomatrix additives bioink additives for 3d bioprinting tissue and organs on 3d bioprinter

One of the largest hurdles of in vitro cell culture has been to mimic conditions that closely resemble in vivo outcomes. Significant strides have been made to this end in the past decades with progress accelerating in more recent years.

One approach that has widely-contributed to progress in tissue engineering and regenerative medicine has been to imitate the human body as accurately as possible. Tissues and organs consist of a milieu of extracellular matrices with varying quantities and placements.

With the advances in 3D bioprinting, scientists now have another tool to move closer to engineering tissues and organs that are more in vivo-like. Employing 3D bioprinting, a variety of ECMs can be precisely deposited in more native formats. 3D bioprinters now have multiple dispensing heads that can simultaneously lay down the ECM with cells in tissue and organ-like configurations. Furthermore, discoveries have recently been made creating native bioinks that are compatible with 3D bioprinters. A combination of such advances and discoveries with 3D bioprinters and native ECM bioinks likely propel future advancements in tissue and organ fabrication.

Further, native collagen bioinks consisting of Type I collagen can also be blended with other ECM’s to formulate more in vivo-like bioinks. Some of these ECMs include Type I, II, III, IV, V collagens, hyaluronic acid, elastin (tropoelastin), fibronectin and vitronectin. ECMs play a major role in achieving the proper cell behavior, cell adhesion signals and binding sites.

In addition to formulating a more optimal ECM environment, cells can be pre-mixed with the bioinks and bioprinted. The cells, in many cases, have been shown to remodel the tissue. Cells secrete and deposit their own intrinsic ECMs, growth factors, cytokines and other biologically relevant components.

The combination of these advanced 3D bioprinters, and cell-laden yet native-to-the-body bioinks, greatly enhance the capabilities and tools available to tissue engineers and scientists.

Allevi is excited to begin offering a broad line of native extracellular matrix proteins from Advanced BioMatrix (ABM) to serve as additives to many of Allevi’s BioInks. Bowman Bagley, Director of Business Development at ABM, comments: “The bar is being raised each day as new publications come out. Researchers are beginning to reject non-native materials as new native, yet printable, bioinks have emerged and are commercially available. The quest to bioprint tissues and organs begins with bioinks composed of native proteins that best replicate a natural, in vivo-like cellular environment. Our goal is to provide all of the proteins that help best replicate the human body when bioprinting. To print native tissues, we need native bioinks.”

As we continue to try and control tissue design, Allevi continues to provide the tools that will allow scientist to most accurately represent human architecture.